Cantharidin induces apoptosis in human bladder cancer TSGH 8301 cells through mitochondria-dependent signal pathways

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Cantharidin induces apoptosis in human bladder cancer TSGH 8301 cells through mitochondria-dependent signal pathways
出版類型SCI(Sciences Citation Index)
出版年度2010
AuthorsYung-Lin Chu, 朱永麟
開始頁1243
頁數7
出版日期2010 / 7
其他編號0000
中文摘要

Cantharidin has shown potent anticancer activities
on many types of human cancer cells. This study was
performed to elucidate whether mitochondria and caspases
are involved in the modulation of apoptosis and cell cycle
arrest by cantharidin in human bladder cancer cells. The
effect of cantharidin on cell cycle arrest, apoptosis, caspases,
reactive oxygen species (ROS) and mitochondrial membrane
potential (Δam) were measured by flow cytometry, and the
levels of apoptosis-associated proteins and its regulatory
molecules were studied by Western blotting. Cantharidininduced
apoptosis and DNA damage was determined by
flow cytometric analysis, DAPI staining and Comet assay.
After cantharidin treatment, the active forms of caspase-3, -8
and -9 were promoted. Cantharidin-induced apoptosis was
associated with enhanced ROS and Ca2+ generations, caused
DNA damage, decreased the levels of Δam and promoted
Endo G and AIF released from mitochondria. Cantharidininduced
G0/G1 arrest was associated with a marked decrease
in the protein expressions of cyclin E and Cdc25c but
promoted the levels of p21 and p-p53. Cantharidin-induced
apoptosis was accompanied with up-regulation of the protein
expression of Bax and PARP, but down-regulation of the
protein levels of Bcl-2,resulting in dysfunction of mitochondria
then led to Endo G and AIF release for causing
induction of apoptosis.

網址http://www.spandidos-publications.com/ijo/37/5/1243
期刊名稱International Journal of Oncology
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