Development of TaqMan probe-based insulated isothermal PCR (iiPCR) for detection of Fusarium oxysporum f. sp. cubense race 4

in International Symposium (poster summary), 國際研討會(摘要海報發表)
標題Development of TaqMan probe-based insulated isothermal PCR (iiPCR) for detection of Fusarium oxysporum f. sp. cubense race 4
出版類型國際研討會(摘要海報發表)
出版年度2015
AuthorsYi-Jia Lin, 林依佳, Tsai-De Chang 張再得, Li-Ling Hong 洪立苓, Tzu-Yu Chen 陳子猷, Jung-Chen Hsu 許榮辰, Pi-Fang Linda Chang 張碧芳, & Ying-Hong Lin 林盈宏
出版日期Aug 4 2015 12:0
會議地點Pasadena, California, USA
其他編號0000
中文摘要

Banana (Musa spp.) is cultivated worldwide and is one of the most popular fruits. Based on the statistics of FAO (Food and Agriculture Organization of the United Nations, Rome, Italy), banana was the third most important fruits in the world. Fusarium wilt of banana caused by F. oxysporum f. sp. cubense (Foc) has become one of the major limiting factors for banana production worldwide. An accurate identification and characterization of pathogens is the basis of integrated pest management of diseases. The lack of rapid methods for disease diagnosis and pathogen detection is one of the main limitations in integrated disease management. Insulated isothermal PCR (iiPCR), established on the basis of the Rayleigh-Benard convective PCR method, is a rapid and low-cost platform for nucleic acid amplification within 60 min. This study developed a novel, efficient detection method based on the TaqMan probe-based iiPCR method for rapid detection of Foc race 4. The iiPCR system is carried out in the R-tubeTM within the POCKITTM Nucleic Acid Analyzer designed by GeneReach Biotechnology Corporation, Taichung, Taiwan. The approach can serve as a rapid, user-friendly, sensitive and specific diagnostic method for in-planta detection of Foc race 4 in field-infected banana.

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