Disease severity and colonization of Fusarium oxysporum f. sp. niveum transformants on watermelon.

in International Symposium (poster summary), 國際研討會(摘要海報發表)
標題Disease severity and colonization of Fusarium oxysporum f. sp. niveum transformants on watermelon.
AuthorsPi-Fang Linda Chang, 張碧芳, Pei-Hsuan Chan 詹佩璇, Ying-Hong Lin 林盈宏, Chien-Tsu Chen 陳建志, Kan-Shu Chen 陳甘澍, & Jenn-Wen Huang 黃振文
出版日期Aug 4 2015 12:0
會議地點Pasadena, California, USA

Fusarium oxysporum f. sp. niveum (Fon) is one of the limiting factors that affect watermelon production. In order to understand the factors affecting disease progress, we transformed Fon with a pPTGFPH binary vector, carrying a stable green fluorescent protein (sGFP), via Agrobacteria tumefaciens. Seven transformants stably expressed green fluorescent protein gene (gfp) were identified. Pathogenicity assays on watermelon seedlings inoculated with spore suspension revealed that two transformants, named Fongfp-PH1 and Fongfp-PH2, induced disease severity at levels similar to that of the wild type Fon H0103 isolate. Three transformants, Fongfp-PH3, Fongfp-PH4 and Fongfp-PH7, displayed a reduced virulence on watermelon compared with wild-type. Fongfp-PH3 and Fongfp-PH4 showed medium disease progress; whereas transformant Fongfp-PH7 showed the mildest symptom. Confocal laser scanning microscopy observation of watermelon inoculated with the Fongfp-PH2 isolate revealed that this isolate produced chlamydospores at 9 days after inoculation (DAI), similar to the wild-type. Transformants Fongfp-PH3 and Fongfp-PH7 produced chlamydospores at 11 and 14 DAI, respectively. Identification of the Fon genes affected by plasmid insertion is currently underway. The flanking genes of the gfp insertion will be further analyzed to understand Fon genes affecting Fusarium wilt disease severity in watermelon.

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