Allicin Induces p53-Mediated Autophagy in Hep G2 Human Liver Cancer Cells

in International Symposium (oral presentation paper), 國際研討會(全文口頭發表)
標題Allicin Induces p53-Mediated Autophagy in Hep G2 Human Liver Cancer Cells
AuthorsYung-Lin Chu, 朱永麟
出版日期May 25 2013 12:0

Garlic has been used throughout history for both culinary and medicinal purpose. Allicin is a major component of
crushed garlic. Although it is sensitive to heat and light and easily metabolized into various compounds such as diallyl disulfide,
diallyl trisulfide, and diallyl sulfide, allicin is still a major bioactive compound of crushed garlic. The mortality of hepatocellular
carcinoma is quite high and ranks among the top 10 cancer-related deaths in Taiwan. Although numerous studies have shown the
cancer-preventive properties of garlic and its components, there is no study on the effect of allicin on the growth of human liver
cancer cells. In this study, we focused on allicin-induced autophagic cell death in human liver cancer Hep G2 cells. Our results
indicated that allicin induced p53-mediated autophagy and inhibited the viability of human hepatocellular carcinoma cell lines.
Using Western blotting, we observed that allicin decreased the level of cytoplasmic p53, the PI3K/mTOR signaling pathway, and
the level of Bcl-2 and increased the expression of AMPK/TSC2 and Beclin-1 signaling pathways in Hep G2 cells. In addition, the
colocalization of LC3-II with MitoTracker-Red (labeling mitochondria), resulting in allicin-induced degradation of mitochondria,
could be observed by confocal laser microscopy. In conclusion, allicin of garlic shows great potential as a novel chemopreventive
agent for the prevention of liver cancer.

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