A set of GFP-based organelle marker lines combined with DsRed-based gateway vectors for subcellular localization study in rice (Oryza sativa L.)

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題A set of GFP-based organelle marker lines combined with DsRed-based gateway vectors for subcellular localization study in rice (Oryza sativa L.)
出版類型SCI(Sciences Citation Index)
出版年度2016
AuthorsTsung-Meng Wu, 吳宗孟, Ke?Chun Lin 林科均, Wei-Shiang Liau 廖偉翔, Yun-Yang Chao 趙雲洋, Ling-Hung Yang 楊翎虹, Szu-Yun Chen 陳思昀, & Chwan?Yang Hong 洪傳揚
開始頁107
頁數8
出版日期2016 / 2
其他編號0000
中文摘要

In the post-genomic era, many useful tools have been developed to accelerate the investigation of gene functions. Fluorescent proteins have been widely used as
protein tags for studying the subcellular localization of proteins in plants. Several fluorescent organelle marker lines have been generated in dicot plants; however, useful and reliable fluorescent organelle marker lines are lacking
in the monocot model rice. Here, we developed eight different GFP-based organelle markers in transgenic rice and created a set of DsRed-based gateway vectors for combining with the marker lines. Two mitochondrial-localized rice ascorbate peroxidase genes fused to DsRed and successfully co-localized with mitochondrial-targeted marker lines verified the practical use of this system. The co-localization of GFP-fusion marker lines and DsRed-fusion proteins provide
a convenient platform for in vivo or in vitro analysis of subcellular localization of rice proteins.

期刊名稱Plant Molecular Biology
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