Effects of sperm pretreatment and embryo activation methods on the development of bovine embryos produced by intracytoplasmic sperm injection

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Effects of sperm pretreatment and embryo activation methods on the development of bovine embryos produced by intracytoplasmic sperm injection
出版類型SCI(Sciences Citation Index)
出版年度2015
AuthorsShyh-Shyan Liu, 劉世賢, Jai-Wei Lee 李嘉偉, & Shen Perng-Chih 沈朋志
開始頁154
頁數8
出版日期2015 / 7
其他編號0000
中文摘要

The aim of the study was to examine the effects of different embryo activation methods and
sperm pretreatments on the activation and development of bovine embryos produced by
intracytoplasmic sperm injection (ICSI). Four activation agents, i.e., calcium ionophore
(A23187), ionomycin (Ion), electric pulse (EP) and ethanol (Eth) were used in various combinations
to activate bovine ICSI embryos. The normal fertilization rate was similar in bovine
ICSI embryos activated by A23187 + Eth, Ion + Eth, Ion + EP + Eth, and 2-Ion (Ion administered
two times) + Eth. Increasing the frequency of ionomycin stimulation from two (2-Ion
+ Eth) to three times (3-Ion + Eth) significantly (p < 0.05) increased the cell number per
embryo at the blastocyst stage. In addition, spermatozoa were pretreated with dithiothreitol
(DTT), glutathione (GSH) or GSH + lysolecithin (LL) and used for producing bovine ICSI
embryos. The blastocyst rate of bovine ICSI embryos produced from sperm pretreated with
GSH was significantly (p < 0.05) higher than those of embryos produced from sperm pretreated
with DTT and GSH + LL. In conclusion, the embryo activation methods and sperm
pretreatments examined in the present study did not affect the normal fertilization rate ofbovine ICSI embryos. However, activation with 3-Ion + Eth and sperm pretreatment with

期刊名稱Reproductive Biology
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