The genome and transcriptome of Phalaenopsis yield insights into floral organ development and flowering regulation

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題The genome and transcriptome of Phalaenopsis yield insights into floral organ development and flowering regulation
出版類型SCI(Sciences Citation Index)
出版年度2016
AuthorsFure-Chyi Chen, 陳福旗
開始頁2017
頁數-2017
出版日期2016 / 5
其他編號A10400022
中文摘要

The Phalaenopsis orchid is an important potted flower of high economic value
around the world. We report the 3.1 Gb draft genome assembly of an important
winter flowering Phalaenopsis ‘KHM190’ cultivar. We generated 89.5 Gb RNA-seq
and 113 million sRNA-seq reads to use these data to identify 41,153 protein-coding
genes and 188 miRNA families. We also generated a draft genome for Phalaenopsis
pulcherrima ‘B8802,’ a summer flowering species, via resequencing. Comparison of
genome data between the two Phalaenopsis cultivars allowed the identification
of 691,532 single-nucleotide polymorphisms. In this study, we reveal that the key
role of PhAGL6b in the regulation of labellum organ development involves
alternative splicing in the big lip mutant. Petal or sepal overexpressing PhAGL6b
leads to the conversion into a lip-like structure. We also discovered that the
gibberellin pathway that regulates the expression of flowering time genes during the
reproductive phase change is induced by cool temperature. Our work thus depicted
a valuable resource for the flowering control, flower architecture development, and
breeding of the Phalaenopsis orchids.

網址http://peerj.com/articles/2017/
期刊名稱Peer J
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