Development of a TaqMan probe-based insulated isothermal polymerase chain reaction (iiPCR) assay for detection of Fusarium oxysporum f. sp. cubense race 4

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Development of a TaqMan probe-based insulated isothermal polymerase chain reaction (iiPCR) assay for detection of Fusarium oxysporum f. sp. cubense race 4
出版類型SCI(Sciences Citation Index)
出版年度2016
AuthorsYing-Hong Lin, 林盈宏, Yi-Jia Lin 林依佳, Tsai-De Chang 張再得, Li-Ling Hong 洪立苓, Tzu-Yu Chen 陳子猷, & Pi-Fang Linda Chang 張碧芳
出版日期2016 / 7
其他編號B10300417
中文摘要

This study developed a novel and inexpensive detection method based on a TaqMan probe-based insulated isothermal polymerase chain reaction (iiPCR) method for the rapid detection of Panama disease caused by Fusarium oxysporum f. sp. cubense (Foc) race 4, which is currently among the most serious fungal vascular diseases worldwide. By using the portable POCKITTM device with the novel primer set iiFoc-1/iiFoc-2, the Foc race 4 iiPCR assay (including DNA amplification and signal monitoring) could be completed within one hour. The developed Foc race 4 iiPCR assay is thus a user-friendly and efficient platform designed specifically for the detection of Foc race 4. The detection limit of this optimized Foc iiPCR system was estimated to be 1 copy of the target standard DNA as well as 1 fg of the Foc genomic DNA. This approach can serve as a rapid detection method for in planta detection of Foc race 4 in field-infected banana. It was concluded that this molecular detection procedure based on iiPCR has good potential for use as an efficient detection method.

期刊名稱PLoS ONE
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