Developing a real-time fluorescence cell growth monitoring system

in International Symposium (oral presentation paper), 國際研討會(全文口頭發表)
標題Developing a real-time fluorescence cell growth monitoring system
AuthorsYao-Nan Wang, 王耀男, & Ko-Tung Chang 張格東
出版日期Aug 18 2015 12:0

CMOS (Complementary Metal-Oxide-Semiconductor) -based real-time fluorescent cells monitoring system can be applied for measuring transfection efficiency or cytotoxicity on pharmacokinetic study. The CMOS sensors have many advantages over traditional microscope CCD (Charge Coupled Device) sensors, including a smaller physical size, a lighter weight, and a lower cost. The aim of the study is to confirm the feasibility and efficiency of our newly developing CMOS-based real-time fluorescent cells monitoring system. We grow CFSE (carboxyfluorescein succinimidyl ester) -labeling RAW264.7 cells in vitro and measure the proliferation rate of these cells by our newly developing instrument and by flow cytometry analysis. CFSE is a fluorescent cell staining dye. This reagent is easily uptake into cells and yields a well-retained fluorescent emission after blue light excitation. When CFSE-labelling cells divide, the mean fluorescent intensity degenerates into half. Thereby, we can real-time detect and tell the distinct generations of proliferating cells and their growth rate by using our new instrument instead of using conventional flow cytometry analysis.

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