Transgenic expression of omega-3 PUFA synthesis genes improves zebrafish survival during Vibrio vulnificus infection

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Transgenic expression of omega-3 PUFA synthesis genes improves zebrafish survival during Vibrio vulnificus infection
出版類型SCI(Sciences Citation Index)
出版年度2016
AuthorsShao-Yang Hu, 胡紹揚, & Jen-Leih Wu 吳金冽
開始頁1
頁數12
出版日期2015 / 11
其他編號0000
中文摘要

Background: Highly desaturated n-3 polyunsaturated fatty acids (PUFAs), such as eicosapentaenoic acid (EPA) and
docosahexaenoic acid (DHA), are synthesized by desaturases and elongase. They exert hepatoprotective effects to
prevent alcoholic fatty liver syndrome or cholestatic liver injury. However, it is unclear how n-3 PUFAs improve
immune function in liver. Vibrio vulnificus, a gram-negative bacterial pathogen, causes high mortality of aquaculture
fishes upon infection. Humans can become infected with V. vulnificus through open wounds or by eating raw
seafood, and such infections may result in systemic septicemia. Moreover, patients with liver diseases are vulnerable
to infection, and are more likely than healthy persons to present with liver inflammation following infection. This
study quantified n-3 PUFAs and their anti-bacterial effects in Fadsd6 and Elvol5a transgenic zebrafish.
Results: Two transgenic zebrafish strains with strong liver specific expression of Fadsd6 and Elvol5a (driven by the
zebrafish Fabp10 promoter) were established using the Tol2 system. Synthesis of n-3 PUFAs in these strains were
increased by 2.5-fold as compared to wild type (Wt) fish. The survival rate in 24 h following challenge with V.
vulnificus was 20 % in Wt, but 70 % in the transgenic strains. In addition, the bacteria counts in transgenic fish
strains were significantly decreased. The expression levels of pro-inflammatory genes, such as TNF-α, IL-1β, and
NF-κB, were suppressed between 9 and 12 h after challenge. This study confirms the anti-bacterial function of n-3
PUFAs in a transgenic zebrafish model.
Conclusions: Fadsd6 and Elvol5a transgenic zebrafish are more resistant to V. vulnificus infection, and enhance
survival by diminishing the attendant inflammatory response.

期刊名稱Journal of Biomedical Science
校址:912 屏東縣內埔鄉學府路1號 總機:886-8-7703202 傳真:886-8-7740165 系統開發統維護單位:國立屏東科技大學 電算中心 版權所有