Dietary supplementation with xylanase-expressing B. amyloliquefaciens R8 improves growth performance and enhances immunity against Aeromonas hydrophila in Nile tilapia (Oreochromis niloticus)

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Dietary supplementation with xylanase-expressing B. amyloliquefaciens R8 improves growth performance and enhances immunity against Aeromonas hydrophila in Nile tilapia (Oreochromis niloticus)
出版類型SCI(Sciences Citation Index)
出版年度2016
AuthorsShao-Yang Hu, 胡紹揚, Yo-Chia Chen 陳又嘉, & Chun-Hung Liu 劉俊宏
開始頁397
頁數8
出版日期2016 / 9
其他編號0000
中文摘要

Bacillus amyloliquefaciens has attracted attention as a probiotic in aquaculture due to its immunostimulatory
activity against pathogenic infection. Xylanases are extensively used in animal feed to degrade
plant ingredients, enhancing nutrient utilization and increasing the growth rate of various animals. In the
present study, the effects of dietary supplementation with B. amyloliquefaciens and xylanase-expressing
B. amyloliquefaciens R8 on the growth of Nile tilapia (Oreochromis niloticus) and immunity against Aeromonas
hydrophila were evaluated. The results showed that the xylanase activity in the intestine, weight
gain (WG), feed efficiency (FE) and condition factor (CF) of Nile tilapia fed B. amyloliquefaciens R8 for 2
months were significantly increased compared with those of the fish fed the control diet and
B. amyloliquefaciens. Moreover, the mRNA expression of growth- and metabolism-related genes, such as
insulin-like growth factor-1 (igf-1), glucokinase (GK), glucose-6-phosphate 1-dehydrogenase (G6PD), and
glucose-6-phosphatase (G6Pase), was significantly induced in Nile tilapia fed administered
B. amyloliquefaciens R8, and this group also exhibited a higher survival rate than the control fish
following a challenge with A. hydrophila. The phagocytic activity and respiratory burst activity of head
kidney leukocytes as well as the serum lysozyme activity of B. amyloliquefaciens R8-fed Nile tilapia were
significantly higher than those of fish fed the control diet for 2 months. Superoxide dismutase (SOD)
levels in the head kidney leukocytes of Nile tilapia fed B. amyloliquefaciens R8 differed from those of fish
fed the control diet, but this was not significant. These results indicate that dietary supplementation with
xylanase-expressing B. amyloliquefaciens R8 improves growth performance and enhances immunity and
disease resistance against A. hydrophila in Nile tilapia.

期刊名稱Fish & Shellfish Immunology
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