Simultaneous Determination of Eleven Quinolones Antibacterial Residues in Marine Products and Animal Tissues by Liquid Chromatography with Fluorescence Detection

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Simultaneous Determination of Eleven Quinolones Antibacterial Residues in Marine Products and Animal Tissues by Liquid Chromatography with Fluorescence Detection
出版類型SCI(Sciences Citation Index)
出版年度2008
AuthorsCHUI-SHIANG CHANG, 張菊香, WEI-HSIEN WANG 王維賢, & Tsai 蔡清恩
開始頁87
頁數9
出版日期2008 / 12
其他編號0000
中文摘要

A simple and efficient multiresidue method was developed for determining 11 quinolones (QNs; marbofloxacin, norfloxacin, ciprofloxacin, lomefloxacin, danofloxacin, enrofloxacin, sarafloxacin, difloxacin, oxolinic acid, nalidixic acid and flumequine) in chicken, pork, fish and shrimp. The analytes were extracted with 0.3% metaphosphoric acid and acetonitrile (1:1, v/v), followed by a HLB cartridge clean-up procedure. The HPLC separation was carried out on a symmetry column C-18 (250 mm × 4.5 mm i.d., 5 μm) with linear gradient elution of 0.1% formic acid and acetonitrile as mobile phase and programmable fluorescence detection. The method was validated by spiking blank animals tissues at three different levels (25, 50 and 250 ng/g; except 6.25, 12.5 and 62.5 ng/g for DAN) while the linearity, detection limit, quantification limit, precision and accuracy were checked. Mean recoveries of 11 QNs from edible animal tissues were 71.7-105.3%. The limits of quantification in different muscle tissues ranged from 5.0 to 28.0 ng/g. The results showed this method was simple, rapid, sensitive and suitable for routine tests.

期刊名稱Journal of Food and Drug Analysis
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