Using a PCR-DGGE Method to Analyze Diazotrophic Diversity in Soil and Root Nodules of Casuarina in the Sihu Area

in English journal, 英文期刊
標題Using a PCR-DGGE Method to Analyze Diazotrophic Diversity in Soil and Root Nodules of Casuarina in the Sihu Area
出版類型英文期刊(EI)
出版年度2017
AuthorsTsung-Po Chang, 張琮柏, Chun-Hsiung Hung 洪俊雄, Yu-Ting Wu 吳羽婷, & Lei-Chen Lin 林瑞進
期刊台灣林業科學
開始頁101
頁數9
出版日期2017 / 6
其他編號0000
中文摘要

Casuarina spp. are major windbreak species in coastal forests of Taiwan. With great ability
for rapid growth and adaptability to adverse environmental conditions, they enhance soil stabilization
and provide protection and rehabilitation for coastal areas. Many factors may limit the
reproduction of Casuarina in coastal areas such as infertile soil, drought, salt stress, litterfall, and
weeds. In addition, nitrogen is generally considered one of the major nutrients in plant growth, and
Casuarina plants can obtain nitrogen sources through association with Frankia via root nodules. In
order to understand the interaction between diverse diazotrophic diversity and different test sites of
Casuarina spp. determine whether it is the diversity level of diazotrophic on healthy and on declining
Casuarina spp., or the number of nitrogen-fixing bacteria in the nodules of Casuarina spp., direct
amplification of the nifH gene for a polymerase chain reaction-denaturing gradient gel electrophoresis
(PCR-DGGE) analysis with the primer pair polF-GC/polR was applied in this study. The
results demonstrated that there were 2 groups of nitrogen-fixing bacteria collected from these root
nodule samples that appeared on the PCR-DGGE profiles. Whether Casuarina stands are on the
decline or not, a high diversity of diazotrophic community has been observed among all test sites.
Therefore, this study shows that the declining Casuarina is not related to the diazotophic diversity.

期刊名稱Taiwan Journal of Forest Science
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