PFLP-intensified disease resistance against bacterial soft rot through MAPK pathway in PAMP-triggered immunity

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題PFLP-intensified disease resistance against bacterial soft rot through MAPK pathway in PAMP-triggered immunity
出版類型SCI(Sciences Citation Index)
AuthorsChuan-Yu Hong, 洪銓佑, Jing-Lin Zheng 鄭靜霖, Tzu-Yi Chen 陳姿誼, How-Ran Chao/Hao-Jan Chao 趙浩然, & Yi-Hsien Lin 林宜賢
出版日期2018 / 7
其他編號MOST 104-2311-B-020-001

Bacterial soft rot is a devastating disease affecting a variety of vegetable crops worldwide. One strategy for controlling this disease could be the ectopic expression of the plant ferredoxin-like protein (PFLP) gene. PFLP was previously shown to intensify Pathogen-Associated Molecular Pattern (PAMP)-triggered immunity (PTI), an immune response triggered, for example, by the flagellin epitope flg22. To gain further insight into how PFLP intensifies PTI, flg22 was used as an elicitor in Arabidopsis thaliana. First, PFLP was confirmed to intensify the rapid generation of H2O2, callose deposition, and the hypersensitive response (HR) when co-infiltrated with flg22. This response correlated with increased expression of the FLG22-induced receptor kinase 1 (FRK1) gene, a gene that is part of the mitogen-activated protein kinase (MAPK) pathway. While the increased response to flg22 alone did not depend on the MAPK pathway genes MEKK1, MKK5, and MPK6, the protective effect of PFLP decreased when plants mutated in these genes were inoculated with Pectobacterium carotovorum subsp. carotovorum. Furthermore, expression of PR1 and PDF1.2 also increased upon treatment with flg22 in the presence of PFLP. Taken together, these results suggest that activation of the MAPK pathway contributes to the increased resistance to bacterial soft rot observed in plants treated with PFLP.

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