Comprehensive insights into composition, metabolic potentials, and interactions among archaeal, bacterial, and viral assemblages in meromictic Lake Shunet in Siberia.

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Comprehensive insights into composition, metabolic potentials, and interactions among archaeal, bacterial, and viral assemblages in meromictic Lake Shunet in Siberia.
出版類型SCI(Sciences Citation Index)
出版年度2018
AuthorsYu-Ting Wu, 吳羽婷, Cheng-Yu Yang C. - Y. Y., Pei-Wen Chiang P. - W. C., Ching-Hung Tseng C. - H. T., Hsiu-Hui Chiu H. - H. C., Isaam Saeed I. S., Bayanmunkh Baatar B. B., Denis Rogozin D. R., Saman Halgamuge S. H., Andrei Degermendzhi A. D., & Sen-Lin Tang S. - L. T.
出版日期2018 / 7
其他編號0000
中文摘要

Microorganisms are critical to maintaining stratified biogeochemical characteristics in
meromictic lakes; however, their community composition and potential roles in nutrient cycling are not thoroughly described. Both metagenomics and metaviromics were used to determine the composition and capacity of archaea, bacteria, and viruses along the water column in the landlocked meromictic Lake Shunet in Siberia. Deep sequencing of 265 Gb and high-quality assembly revealed a near-complete genome corresponding to Nonlabens sp. in a viral sample and 38 bacterial bins (0.2–5.3 Mb each). The mixolimnion (3.0 m) had the most diverse archaeal, bacterial, and viral communities, followed by the monimolimnion (5.5 m) and chemocline (5.0 m). The bacterial and archaeal communities were dominated by Thiocapsa and Methanococcoides, respectively, whereas the viral community was dominated by Siphoviridae. The archaeal and bacterial assemblages and the associated energy metabolism were significantly related to the various depths, in accordance with the stratification of physicochemical parameters. Reconstructed elemental nutrient cycles of the three layers were interconnected, including co-occurrence of denitrification and nitrogen fixation in each layer and involved unique processes due to specific biogeochemical properties at the respective depths. Notably, several predominant yet unknown and uncultured bacteria were potentially capable of nutrient cycling. Reciprocal BLAST analysis revealed that the viruses were specific to the host archaea and bacteria in the mixolimnion. This study provides insights into the bacterial, archaeal, and viral assemblages and the corresponding capacity potentials in Lake Shunet, one of the three meromictic lakes in central Asia. Lake Shunet was determined to harbor specific and diverse viral, bacterial, and archaeal communities that intimately interacted, revealing patterns shaped by indigenous physicochemical parameters.

期刊名稱Frontiers in Microbiology
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