Methods for efficient analysis of tocopherols, tocotrienols and their metabolites in animal samples with HPLC-EC

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Methods for efficient analysis of tocopherols, tocotrienols and their metabolites in animal samples with HPLC-EC
出版類型SCI(Sciences Citation Index)
出版年度2018
AuthorsMao-Jung Lee, 李茂榮, William Feng 馮威廉, Lu Yang 楊璐, Yu-Kuo Chen 陳與國, Eric Chi 季艾瑞克, Anna Ba Liu 劉八, & Chung S. Yang 楊中樞
開始頁318
頁數11
出版日期2018 / 1
其他編號0000
中文摘要

Tocopherols and tocotrienols, collectively known as vitamin E, have received a great deal of attention because of their interesting biological activities. In the present study, we reexamined and improved previous methods of sample preparation and the conditions of high-performance liquid chromatography for more accurate quantification of tocopherols, tocotrienols and their major chain-degradation metabolites. For the analysis of serum tocopherols/tocotrienols, we reconfirmed our method of mixing serum with ethanol followed by hexane extraction. For the analysis of tissue samples, we improved our methods by extracting tocopherols/tocotrienols directly from tissue homogenate with hexane. For the analysis of total amounts (conjugated and unconjugated forms) of side-chain degradation metabolites, the samples need to be deconjugated by incubating with β-glucuronidase and sulfatase; serum samples can be directly used for the incubation, whereas for tissue homogenates a pre-deproteination step is needed. The present methods are sensitive, convenient and are suitable for the determination of different forms of vitamin E and their metabolites in animal and human studies. Results from the analysis of serum, liver, kidney, lung and urine samples from mice that had been treated with mixtures of tocotrienols and tocopherols are presented as examples.

網址https://www.sciencedirect.com/science/article/pii/S1021949817301497
期刊名稱Journal of Food and Drug Analysis
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