Arsenic inhibits induction of cytochrome P450 1A1 by 2,3,7,8-tetrachlorodibenzo-p-dioxin in human hepatoma cells | Faculty Open Information System

Arsenic inhibits induction of cytochrome P450 1A1 by 2,3,7,8-tetrachlorodibenzo-p-dioxin in human hepatoma cells

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Arsenic inhibits induction of cytochrome P450 1A1 by 2,3,7,8-tetrachlorodibenzo-p-dioxin in human hepatoma cells
出版類型SCI(Sciences Citation Index)
出版年度2006
AuthorsHow-Ran Chao/Hao-Jan Chao, 趙浩然
開始頁716
頁數6
出版日期2006 / 9
其他編號0000
中文摘要

The aim of this study was to examine the arsenic effect on activation of aryl hydrocarbon receptor (AhR)-mediated gene expression by
2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) in human hepatoma cells. The human hepatoma Huh7 cells were treated with sodium arsenite
(NaAsO2) from 0.5 to 20 Mfor 24 h. Our data revealed that NaAsO2 ?10Mcaused no significant cytotoxic effect on Huh7 cells (p > 0.05).We
also established a dioxin-responsive element (DRE)-mediated Chemical Activated LUciferase eXpression (CALUX) cell line, Huh7-DRE-Luc, by
stable transfection of Huh7 with a DRE-driven firefly luciferase reporter plasmid (4xDRE-TATA-Luc). Treatments of Huh7-DRE-Luc and Huh7
with NaAsO2 attenuated the 2,3,7,8-TCDD-induced DRE-CALUX and cytochrome P450 1A1 (CYP1A1) activations, respectively, in a dosedependent
manner. We found that the calculated CALUX-toxic equivalent (TEQ) levels induced by cotreatment of NaAsO2 ?3.0M and 10nM
2,3,7,8-TCDD were significantly lower than that induced by 2,3,7,8-TCDD alone (p < 0.05). In the present study, we demonstrated that arsenic not
only inhibited the TCDD-induced CYP1A1 activation but also interfered with DRE-CALUX bioassay in human hepatoma cells. Our finding also
suggests that extensive cleanup of sample for removal of any possible interfering factor is critical to guarantee the accuracy of dioxin-TEQ levels
using DRE-CALUX bioassay

期刊名稱Journal of Hazardous Materials
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