Vibrio alginolyticus infection confirmed by polymerase chain reaction and 16S rDNA sequencing in the white shrimp Litopenaeus vannamei

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Vibrio alginolyticus infection confirmed by polymerase chain reaction and 16S rDNA sequencing in the white shrimp Litopenaeus vannamei
出版類型SCI(Sciences Citation Index)
出版年度2004
AuthorsChun-Hung Liu, 劉俊宏, Winton Cheng 鄭文騰, Hsu C 徐榮彬, & J. C. Chen 陳建初
開始頁169
頁數5
出版日期2004 / 10
其他編號0000
中文摘要

A Gram-negative, rod-shaped bacterium identified as Vibrio alginolyticus was isolated from diseased Litopenaeus vannamei (also called Penaeus vannamei) in Taiwanese culture ponds. The diseased shrimp displayed poor growth, anorexia, inactivity, reddish pleural borders of antennae, uropods and telson, opaque and whitish musculature, and mortality. In histological preparations, melanized hemocytic granulomas were observed in the connective tissue around hemal sinuses
together with hemocytic aggregation in necrotic musculature. Six isolates of Vibrio were collected from diseased shrimp at 3 farms, and these were evaluated for characteristics including morphology, physiology, biochemistry and sensitivity to antibiotics. The results indicated that the isolates belonged to a single species that grew in 1 to 8% NaCl, at 10 to 40°C and on TCBS (thiosulfatecitrate-
bile sucrose) agar, and that gave positive catalase, O/F (Oxidation/Fermentation), lysine decarboxylase, gelatinase and cytochrome-oxidase tests. Identification of CH003 (1 of 6 isolates) was confirmed by PCR assay for V. alginolyticus (expected amplicon 1486 bp). The 16S rDNA sequence (GenBank accession number AY373027) gave 99.9% sequence identity to V. alginolyticus (GenBank accession number X74690). The calculated 96 h LD50 dose of the isolated strain was 3.0 × 105 colony forming units (CFU) shrimp–1 (6.6 × 104 CFU g–1).

期刊名稱Dis. Aquat. Org.
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